منابع مشابه
Glucose transport in human red blood cells
The penetration of D-[C] glucose into human red blood cells (RBCs) features kinetic parameters which are readily distinguishable from passive permeation. It would be expected to require activation energy above 80 kJ/mol for permeation of glucose with five hydroxyls capable of forming hydrogen bonds, but the measured activation energy is approximately 16 kJ/mol. As a consequence, glucose permeat...
متن کاملRed blood cell lactate transport in sickle disease and sickle cell trait.
This study determined and compared rates and mechanisms of lactate transport in red blood cells (RBCs) of persons with 1) sickle cell disease (HbSS), 2) sickle cell trait (HbAS), and 3) a control group (HbAA). Blood samples were drawn from 30 African-American volunteers (10 HbSS, 10 HbAS, 10 HbAA). Lactate influx into RBCs was measured by using [14C]lactate at six (2, 5, 10, 15, 25, and 40 mM) ...
متن کاملImpaired vasodilation by red blood cells in sickle cell disease.
Red blood cells (RBCs) have been ascribed a unique role in dilating blood vessels, which requires O2-regulated binding and bioactivation of NO by Hb and transfer of NO equivalents to the RBC membrane. Vasoocclusion in hypoxic tissues is the hallmark of sickle cell anemia. Here we show that sickle cell Hb variant S (HbS) is deficient both in the intramolecular transfer of NO from heme iron (iron...
متن کاملDynamic deformability of sickle red blood cells in microphysiological flow.
In sickle cell disease (SCD), hemoglobin molecules polymerize intracellularly and lead to a cascade of events resulting in decreased deformability and increased adhesion of red blood cells (RBCs). Decreased deformability and increased adhesion of sickle RBCs lead to blood vessel occlusion (vaso-occlusion) in SCD patients. Here, we present a microfluidic approach integrated with a cell dimension...
متن کاملCytosolic free calcium levels in sickle red blood cells.
In this study, we used a recently developed nuclear magnetic resonance (NMR) technique to measure ionized calcium in sickle erythrocytes. The NMR technique, which involves 19F NMR studies of a fluorinated calcium chelator quinMF, [2-(2-amino-4-methyl-5-fluorophenoxy)methyl-8-aminoquinoline-N,N,N',N'- tetraacetic acid] provides a novel approach to the study of ionized calcium in erythrocytes sin...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1990
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-199012000-00008